Anti-Phospho-CaM Kinase II (Thr286) Camk2a Antibody

Product Name

Anti-Phospho-CaM Kinase II (Thr286) Camk2a Antibody

View all Camk2a Antibodies

SKU/Catalog Number

P03241

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-CaM Kinase II (Thr286) Camk2a Antibody (Catalog # P03241). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rabbit, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-CaM Kinase II (Thr286) Camk2a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03241)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

608

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P03241 is reactive to Camk2a in Human, Mouse, Rabbit, Rat

Applications

P03241 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

54.115kDa

Background of Camk2a

Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr-286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr-305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:1000

Validation Images & Assay Conditions

Click image to see more details

Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa alpha-and the ~60 kDa beta-CaM Kinase II phosphorylated at Thr²⁸⁶ in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 minutes).

Gene/Protein Information For Camk2a (Source: Uniprot.org, NCBI)

Alternative Names

calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; CaM kinase II alpha subunit; CaM Kinase II alpha; CaM kinase II subunit alpha; CAMK2A; CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha; CaMK-II alpha subunit; CaMK-II subunit alpha; CaMKIINalpha; CaM-kinase II alpha chain; EC 2.7.11; EC 2.7.11.17; KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain Camk2a|PK2CDD, PKCCD|calcium/calmodulin-dependent protein kinase II alpha|calcium/calmodulin-dependent protein kinase type II subunit alpha|Ca2+/calmodulin-dependent protein kinase II alpha|alpha CaM kinase II|caM kinase II subunit alpha|caM-kinase II alpha chain|caMK-II subunit alpha|calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha|calcium/calmodulin-dependent protein kinase II alpha subunit|calcium/calmodulin-dependent protein kinase type II alpha chain

For more info on Camk2a, check out the Camk2a Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Camk2a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the Camk2a Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis